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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Size (kb): 3.0269999504089360
Vector: pDK101 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pGEM-5Zf(+), synthetic adaptor
Construct size (kb): 3.026999950408936
Features: insert detection: lacZ'
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0.
The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014.
A high copy number vector useful for direct cloning of double stranded amplification products.
Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products.
Kovalic D, et al. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19: 4560, 1991. PubMed: 1886782