pDK101 (ATCC® 77406)

Applications: vector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector useful for cloning PCR products  /  Depositors: B Weisblum

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Designations pDK101
Depositors B Weisblum
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.0269999504089360
Vector: pDK101 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pGEM-5Zf(+), synthetic adaptor
Construct size (kb): 3.026999950408936
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0.
The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014.
A high copy number vector useful for direct cloning of double stranded amplification products.
Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Kovalic D, et al. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19: 4560, 1991. PubMed: 1886782

Shipped frozen
Product Sheet
Product Sheet
Product Sheet
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