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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 3.0269999504089360 Vector: pDK101 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pGEM-5Zf(+), synthetic adaptor Marker(s):ampR Construct size (kb): 3.026999950408936 Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI
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Applications
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vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector useful for cloning PCR products
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Comments
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Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. A high copy number vector useful for direct cloning of double stranded amplification products. Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products.
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References
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Kovalic D, et al. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19: 4560, 1991. PubMed: 1886782
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