pDK101 (ATCC® 77406)

Applications: vector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector useful for cloning PCR products  /  Depositors: B Weisblum

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Designations pDK101
Depositors B Weisblum
Biosafety Level 1
Vector Information
Size (kb): 3.0269999504089360
Vector: pDK101 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pGEM-5Zf(+), synthetic adaptor
Marker(s):ampR
Construct size (kb): 3.026999950408936
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI
Applications
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector useful for cloning PCR products
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0.
The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014.
A high copy number vector useful for direct cloning of double stranded amplification products.
Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Kovalic D, et al. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19: 4560, 1991. PubMed: 1886782

Shipped frozen
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