pdeltablue (ATCC® 77334)

Applications: expression vectorvector permitting visual detection of recombinants  /  Depositors: ME Brandt

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Designations pdeltablue
Depositors ME Brandt
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5069999694824220
Vector: pdeltablue (plasmid)
Promoters: Promoter lambda PL
Construction: pfXblue
Construct size (kb): 3.506999969482422
Features: insert detection: lacZ'
marker(s): ampR
promoter: lambda PL
replicon: pMB1
expression vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45.
Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25.
Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon.
The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon.
Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.
Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943).
The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Brandt ME, Vickery LE. Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli. Arch. Biochem. Biophys. 294: 735-740, 1992. PubMed: 1567230

Mark E Brandt, personal communication

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