pdeltablue (ATCC® 77334)

Applications: expression vectorvector permitting visual detection of recombinants  /  Depositors: ME Brandt

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Designations pdeltablue
Depositors ME Brandt
Biosafety Level 1
Vector Information
Size (kb): 3.5069999694824220
Vector: pdeltablue (plasmid)
Promoters: Promoter lambda PL
Construction: pfXblue
Marker(s):ampR
Construct size (kb): 3.506999969482422
Features: insert detection: lacZ'
marker(s): ampR
promoter: lambda PL
replicon: pMB1
Applications
expression vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45.
Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25.
Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon.
The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon.
Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.
Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943).
The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brandt ME, Vickery LE. Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli. Arch. Biochem. Biophys. 294: 735-740, 1992. PubMed: 1567230

Mark E Brandt, personal communication

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