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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 3.5069999694824220 Vector: pdeltablue (plasmid) Promoters: Promoter lambda PL Construction: pfXblue Marker(s):ampR Construct size (kb): 3.506999969482422 Features: insert detection: lacZ'
marker(s): ampR
promoter: lambda PL
replicon: pMB1
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Applications
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expression vector vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45. Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25. Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon. The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon. Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies. Constructed from pfXblue ( ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943). The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'.
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References
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Brandt ME, Vickery LE. Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli. Arch. Biochem. Biophys. 294: 735-740, 1992. PubMed: 1567230
Mark E Brandt, personal communication
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