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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.9840002059936520 Vector: pGEX-KT (plasmid) Promoters: Promoter tac Construction: pGEX-1 Marker(s):ampR Construct size (kb): 4.984000205993652 Features: marker(s): ampR
other: thrombin cleavage site
promoter: tac
replicon: pMB1
repressor gene: lacIq
MCS: BamHI SmaI EcoRI
epitope tag: GST
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Applications
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encodes an epitope tag for protein isolation or monitoring expression vector vector permitting construction of fusion proteins
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI/PstI--4.0, 1.0; EcoRI/EcoRV--3.2, 1.8. The resulting protein contains two additional amino acids at the amino terminus (Gly-Ser). Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids. The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine "kinker" and thrombin recognition site to enhance cleavage of the fusion protein.
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References
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Hakes DJ, Dixon JE. New vectors for high level expression of recombinant proteins in bacteria. Anal. Biochem. 202: 293-298, 1992. PubMed: 1519755
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Shipping Information
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Freeze dried E. coli containing the plasmid
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