pUN90 [pNN338] (ATCC® 77269)

Applications: YC-type (centromeric) shuttle vectormutation detectionshuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pUN90 [pNN338]
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 5.5999999046325680
Vector: pUN90 (plasmid)
Promoters: Promoter lac
Construction: pUC18
Marker(s):ampR,HIS3
Construct size (kb): 5.599999904632568
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
mutation detection
shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; SacI--5.6; SalI--5.6.
YC-type shuttle vector permitting visual detection of recombinants by lacZalpha complementation.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604

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