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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.8010001182556150 Vector: pFL59- (phagemid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.801000118255615 Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac
replicon: pMB1, f1, ARS-CEN6
centromere: CEN6
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Applications
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YC-type (centromeric) shuttle vector shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori.
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References
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Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827
Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361
Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282
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