pECV25 (ATCC® 77187)

Applications: contains sequence nuclear antigen 1expression vectorshuttle vector  /  Depositors: C Backendorf

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Designations pECV25
Depositors C Backendorf
Biosafety Level 1
Vector Information
Size (kb): 11.3170003890991200
Vector: pECV25 (plasmid)
Promoters: Promoter RSV LTR
Construction: pRSVneo, p205
Marker(s):G418R,ampR,hygR
Construct size (kb): 11.31700038909912
Features: marker(s): ampR, hygR, G418R
promoter: RSV LTR
replicon: pMB1, EBV oriP
terminator: beta-globin polyadenylation
Applications
contains sequence nuclear antigen 1
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/SacI--5.7 (doublet); SalI--11.5; XhoI--11.5.
Shuttle expression vector maintained episomally in human cells at a copy number of 25 - 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids.
Vector can be rescued from transfected cells with no detectable rearrangements.
The EBNA-1 gene contains an in-frame deletion of a repetitive region of 700 +/- 20 bp. This deletion increases the number of stably maintained plasmid molecules per cell.
The order of the major features in this plasmid is: bla (ampR) - hph (hygR) - RSV LTR - HindIII - rabbit beta-globin intron splicing signals - NotI - agpt (G418R) - XhoI - rabbit beta-globin polyadenylation signal - SalI - oriP - SacI - EBNA-1 - ClaI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Belt PB, et al. Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector. Nucleic Acids Res. 19: 4861-4866, 1991. PubMed: 1656380

Belt PB, et al. Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene. Nucleic Acids Res. 19: 5633-5637, 1991. PubMed: 1945841

Belt PB, et al. Construction and properties of an Epstein-Barr-virus-derived cDNA expression vector for human cells. Gene 84: 407-417, 1989. PubMed: 2482230

Shipped frozen
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