pCMVbeta (ATCC® 77177)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorreporter constructvector permitting visual detection of activity in colonies  /  Depositors: GR Macgregor

Permits and Restrictions

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Designations pCMVbeta
Depositors GR Macgregor
Biosafety Level 1
Vector Information
Size (kb): 7.1640000343322750
Vector: pCMVbeta (plasmid)
Promoters: Promoter CMV immediate early
Construction: pUC19, pC4AUG beta-gal, pCM5029, pL1
Marker(s):ampR
Construct size (kb): 7.164000034332275
Features: eukaryotic promoter/expression cassette: CMV promoter/enhancer SV40 splice signal
eukaryotic reporter gene cassette: lacZ
marker(s): ampR
promoter: CMV immediate early
replicon: pMB1
reporter group: lacZ
terminator: SV40 late
enhancer: CMV immediate early
Applications
contains easily purifiable cassette(s) for construction
expression vector
reporter construct
vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2; NotI/EcoRI--3.54, 2.9, 0.9.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
The order of the major features in this plasmid is: pUC19 - EcoRI - PstI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - PstI - HindIII - pUC19.
Media Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524

Shipped freeze-dried
Shipping Information Freeze dried E. coli containing the plasmid
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 77177