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pCMVbeta (ATCC^® 77177^™)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorreporter constructvector permitting visual detection of activity in colonies / Depositors: GR Macgregor

pCMVbeta ATCC^® 77177^™

freeze-dried

For-Profit: $290.00 Non-Profit: $290.00

Qty:

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pCMVbeta
Depositors	GR Macgregor
Biosafety Level	1
Vector Information	Size (kb): 7.1640000343322750 Vector: pCMVbeta (plasmid) Promoters: Promoter CMV immediate early Construction: pUC19, pC4AUG beta-gal, pCM5029, pL1 Marker(s):ampR Construct size (kb): 7.164000034332275 Features: eukaryotic promoter/expression cassette: CMV promoter/enhancer SV40 splice signal eukaryotic reporter gene cassette: lacZ marker(s): ampR promoter: CMV immediate early replicon: pMB1 reporter group: lacZ terminator: SV40 late enhancer: CMV immediate early
Applications	contains easily purifiable cassette(s) for construction expression vector reporter construct vector permitting visual detection of activity in colonies
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2; NotI/EcoRI--3.54, 2.9, 0.9. Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. The lacZ gene may be excised by NotI and replaced with alternative sequences. The order of the major features in this plasmid is: pUC19 - EcoRI - PstI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - PstI - HindIII - pUC19.
Media	ATCC^® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524
Shipped	FD
Shipping Information	Freeze dried E. coli containing the plasmid

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector map for 77177