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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.5040001869201660 Vector: pRS305 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRSS56 [pBluescript KS+, pBS(+)] Marker(s):LEU2,ampR Construct size (kb): 5.504000186920166 Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: SacI...KpnI
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Applications
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YI-type (integrating) shuttle vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): HindIII--5.6; PvuII--5.1, 0.46; KpnI--3.8, 1.7; EcoRI--3.5, 2.0. One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (2.235 kb) containing the LEU2 gene was inserted into the NdeI site of pRSS56. All ends were blunted. The order of the major features in this plasmid is: LEU2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla. The following restriction sites in the multiple cloning site (MCS) are no longer unique: KpnI ClaI EcoRV EcoRI BstXI.
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References
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Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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Shipping Information
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Freeze dried phagemid in E. coli
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