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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector
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Construct size (kb): 5.400000095367432
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Insert
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DNA: cDNA Insert lengths(kb): 2.400000095367432 Gene product: wingless-type MMTV integration site 1, human homolog [Int1]
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Shipping Information
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Distributed: freeze-dried
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Comments
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Insert contains internal BamHI (1) and ClaI (1) sites. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 2.4; EcoRI--5.4; HindIII--5.4; BamHI--5.4; consistent with deposited DNA. A new nomenclature system has been proposed for Int-1 and related genes. It has been suggested that Int-1 become Wnt-1. Contains cDNA of murine int-1 that encodes the complete protein. RNA can be synthesized in vitro from the SP6 vector promoter.
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Classification
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Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
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References
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van Ooyen A, Nusse R. Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact. Cell 39: 233-240, 1984. PubMed: 6091914
Rijsewijk FA, et al. Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro. Nucleic Acids Res. 14: 693-702, 1986. PubMed: 3003692
Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319
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