47z [p47z] (ATCC® 61024)

Clone Type: Clone  / 

Permits and Restrictions

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Designations 47z [p47z]
Construct size (kb): 5.699999809265137
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: DNA Segment, single copy, homologous on X and Y [DXYS5]
Alleles: B1, A1, A2, B1, A1, A2, A1, B2, C1, C2, A1, A2, B2, C1, C2, A2
Insert Size (kb) 2.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.4; EcoRI/BamHI--2.9, 2.3, 0.39; BamHI--5.7; PstI--5.7; PvuI--5.7.
EcoRI detects a 2.6 kb band common to X and Y chromosomes. TaqI detects a 4.3 kb Y-specific band and X-specific bands of 3.2 and 1.5 kb.
The HindIII/TaqI polymorphism has demonstrated co-dominant segregation in 8 families.
The StuI polymorphism demonstrated Y-linked inheritance in 6 families.
Wash in 2X SSC at 68C.

Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206

Geldwerth D, et al. Extensive DNA sequence homologies between the human Y and the long arm of the X chromosome. EMBO J. 4: 1739-1743, 1985. PubMed: 4029125

Nakahori Y, et al. Two 47z [DXYS5] RFLPs on the X and the Y chromosome. Nucleic Acids Res. 17: 2152, 1989. PubMed: 2564668

Jean Weissenbach, personal communication

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