47z [p47z] (ATCC® 61024)

Clone Type: Clone  / 

Permits and Restrictions

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Designations 47z [p47z]
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: DNA Segment, single copy, homologous on X and Y [DXYS5]
Alleles: B1, A1, A2, B1, A1, A2, A1, B2, C1, C2, A1, A2, B2, C1, C2, A2
Insert Size (kb) 2.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.4; EcoRI/BamHI--2.9, 2.3, 0.39; BamHI--5.7; PstI--5.7; PvuI--5.7.
EcoRI detects a 2.6 kb band common to X and Y chromosomes. TaqI detects a 4.3 kb Y-specific band and X-specific bands of 3.2 and 1.5 kb.
The HindIII/TaqI polymorphism has demonstrated co-dominant segregation in 8 families.
The StuI polymorphism demonstrated Y-linked inheritance in 6 families.
Wash in 2X SSC at 68C.
References

Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206

Geldwerth D, et al. Extensive DNA sequence homologies between the human Y and the long arm of the X chromosome. EMBO J. 4: 1739-1743, 1985. PubMed: 4029125

Nakahori Y, et al. Two 47z [DXYS5] RFLPs on the X and the Y chromosome. Nucleic Acids Res. 17: 2152, 1989. PubMed: 2564668

Jean Weissenbach, personal communication

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