Spongomonas minima Dangeard (ATCC® 50405™) Organism: Spongomonas minima Dangeard / Depositor: TA Nerad General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. This material is distributed for research purposes only and is part of the National Park Service Special Collection. A signed MTA is required prior to shipment for non-commercial customers. A licensing agreement is required from the National Park Service for commercial customers. Strain Designations UT-1 Biosafety Level 1 Isolation sediment, freshwaterUtah, United StatesIsolation date: 1988 Product Format dried Type Strain no Comments Very similar to ATCC 50404, probably the same species. This strain is tentatively placed in this genus because the cells superficially resemble members of Spongomonas based upon features discernable at the light microscopical level.The strain will probably be assigned to a new genus. Medium ATCC® Medium 802: Sonneborn's Paramecium medium Growth Conditions Temperature: 25.0°CProtocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium. Subcultivation Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium. Name of Depositor TA Nerad Special Collection National Park Service Special Collection (NPS) Geographical Isolation Arches National Park Year of Origin 1988 References dry river bed of Courthouse Wash, Arches National Park, Grand Co, UT Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. This material is distributed for research purposes only and is part of the National Park Service Special Collection. A signed MTA is required prior to shipment for non-commercial customers. A licensing agreement is required from the National Park Service for commercial customers. Basic Documentation Product Sheet Certificate of Analysis MSDS