Spongomonas minima Dangeard (ATCC® 50405)

Organism: Spongomonas minima Dangeard  /  Depositor: TA Nerad

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Strain Designations UT-1
Biosafety Level 1
Isolation
sediment, freshwater
Utah, United States
Isolation date: 1988
Product Format dried
Type Strain no
Comments
Very similar to ATCC 50404, probably the same species. This strain is tentatively placed in this genus because the cells superficially resemble members of Spongomonas based upon features discernable at the light microscopical level.
The strain will probably be assigned to a new genus.
Medium Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium.
Subcultivation
Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium.
Name of Depositor TA Nerad
Special Collection National Park Service Special Collection (NPS)
Geographical Isolation Arches National Park
Year of Origin 1988
References

dry river bed of Courthouse Wash, Arches National Park, Grand Co, UT

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • This material is distributed for scientific and educational research purposes only and is part of the National Park Service Special Collection. A signed National Park Service MTA is both required prior to shipment to customers proposing a non-commercial use. A separate agreement is required from the National Park Service for customers proposing a commercial use
Basic Documentation