Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47076)

Strain Designations: MG1655  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations MG1655
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Shipping Information Frozen gycerol stock of E. coli
Storage Conditions -80°C or colder
Preceptrol® no
Genotype F- lambda-
Type Strain no
Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: and
Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis.
AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096
Derived from the parent strain W1485 by curing of the F plasmid.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Genotype F- lambda-
Name of Depositor GM Weinstock
Chain of Custody
ATCC <-- GM Weinstock <-- MG1655

Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357

Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503

Burland V, et al. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16: 551-561, 1993. PubMed: 7686882

Daniels DL, et al. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257: 771-778, 1992. PubMed: 1379743

Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124

Heath JD, et al. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J. Bacteriol. 174: 558-567, 1992. PubMed: 1729244

Perkins JD, et al. XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J. Mol. Biol. 232: 419-445, 1993. PubMed: 8393935

Plunkett G III, et al. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 21: 3391-3398, 1993. PubMed: 8346018

Johanson U, Hughes D. Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli. Gene 120: 93-98, 1992. PubMed: 1398129

Jensen KF. The Escherichia coli K-12 'wild types' W3110 and MG1655 have an rphframeshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Gene 175: 3401-3407, 1993. PubMed: 8501045

Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388

Cross References

Nucleotide (GenBank) : U00096 complete sequence

Nucleotide (GenBank) : X72920 intercistronic region

Nucleotide (GenBank) : X64592 gene for ribosomal protein S7, partial

Nucleotide (GenBank) : L10328 chromosomal region from 81.5 to 84.5 minutes

Nucleotide (GenBank) : L19201 chromosomal region from 87.2 to 89.2 minutes

Nucleotide (GenBank) : M87049 chromosomal region from 84.5 to 86.5 minutes

Nucleotide (GenBank) : U00006 chromosomal region from 89.2 to 92.8 minutes

Nucleotide (GenBank) : X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3' end)