Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47024)

Strain Designations: BNN93 [C600]  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations BNN93 [C600]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli.

Storage Conditions

Conditions: Store unopened frozen vial at -80°C.

Preceptrol® no
Genotype F- hsdR supE44 thr-1 leuB6 thi-1 lacY1 fhuA21 e14- mcrB lambda-
Type Strain no
Comments
A general purpose host strain suitable for lambdagt10.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- hsdR supE44 thr-1 leuB6 thi-1 lacY1 fhuA21 e14- mcrB lambda-
Name of Depositor R Young, RW Davis
References

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Raleigh EA, et al. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 16: 1563-1575, 1988. PubMed: 2831502

Woodcock DM, et al. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17: 3469-3478, 1989. PubMed: 2657660

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation