Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47000)

Strain Designations: NM522 [71-18 hsddelta5]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations NM522 [71-18 hsddelta5]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Shipping Information

Shipped:  Freeze dried E. coli

Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F' lacIq delta(lacZ)M15 proA+ proB+ delta(hsdMS-mcrB)5 delta(lac-proAB) supE thi-1 lambda-
Type Strain no
Comments
A lacZ host lacking a restriction system for M13 and other filamentous bacteriophage.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F' lacIq delta(lacZ)M15 proA+ proB+ delta(hsdMS-mcrB)5 delta(lac-proAB) supE thi-1 lambda-
Name of Depositor D Mead
References

Gough JA, Murray NE. Sequence diversity among related genes for recognition of specific targets in DNA molecules. J. Mol. Biol. 166: 1-19, 1983. PubMed: 6304321

Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795