|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Designations
|
pHPV-16 plasmid in E. coli
|
|
GenBank Definition
|
Human papillomavirus type 16 (HPV16), complete genome
|
|
Species
|
human papilloma virus type 16
|
|
Vector
|
Construct size (kb): 10.86
|
|
Insert
|
DNA: genomic Insert lengths(kb): 7.904 Gene product: human papilloma virus genome [HPV-16]
|
|
Related Products
|
Purified plasmid DNA: ATCC 45113D |
|
Comments
|
Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site Full-length. The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI - 685 [6818], 1320 [7453]. BamHI+BglI will excise the insert with 205 nucleotides of the vector. Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6.
|
|
Classification
|
Papovavirus, Papillomavirus, recombinant
|
|
References
|
James E Marich, personal communication
|