pF4 plasmid in E. coli (ATCC® 45010)

Organism: Moloney murine sarcoma virus, Moloney murine sarcoma virus  /  Clone Type: Clone  /  Depositors: UA Rapp

Permits and Restrictions

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Designations pF4 plasmid in E. coli
Species Moloney murine sarcoma virus, Moloney murine sarcoma virus
Depositors UA Rapp
Vector
Construct size (kb): 13.2
Insert
DNA: genomic
Insert lengths(kb): 8.9
Tissue: transformed Fischer rat embryo FRE 3A cell line
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1 pseudogene 1 [RAF1]
Alleles: A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, B1, A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, A1, A2, B2
Insert Size (kb) 8.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): SalI--14.0, HindIII--9.0, 4.5; EcoRI--9.4, 4.4.
Integrated proviral DNA FRE-3A. Fisher rat cells infected with 3611-MSV. Constructed by inserting 8.9 Kbp EcoRI fragment containing 3611-MSV
This clone transforms transfected 3T3 cells and induces sarcoma in NFS/N mice.
Includes about 50 bp of 5' and 1.3 kb of 3' flanking cellular (rat FRE-3A) sequences.
The v-raf gene is contained in a 1.5 kb XhoI/BglII fragment of the insert.
Classification Retroviridae, Gammaretrovirus, Mammalian virus group
References

Rapp UR, et al. New mammalian transforming retrovirus: demonstration of a polyprotein gene product. J. Virol. 45: 914-924, 1983. PubMed: 6300462

Bonner T, et al. The human homologs of the raf (mil) oncogene are located on human chromosomes 3 and 4. Science 223: 71-74, 1984. PubMed: 6691137

Rapp UR, et al. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Natl. Acad. Sci. USA 80: 4218-4222, 1983. PubMed: 6308607