pF4 plasmid in E. coli (ATCC® 45010)

Organism: Moloney murine sarcoma virus, Moloney murine sarcoma virus  /  Clone Type: Clone  /  Depositors: UA Rapp

Permits and Restrictions

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Designations pF4 plasmid in E. coli
Species Moloney murine sarcoma virus, Moloney murine sarcoma virus
Depositors UA Rapp
Vector
Construct size (kb): 13.2
Insert
DNA: genomic
Insert lengths(kb): 8.9
Tissue: transformed Fischer rat embryo FRE 3A cell line
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1 pseudogene 1 [RAF1]
Alleles: A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, B1, A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, A1, A2, B2
Insert Size (kb) 8.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): SalI--14.0, HindIII--9.0, 4.5; EcoRI--9.4, 4.4.
Integrated proviral DNA FRE-3A. Fisher rat cells infected with 3611-MSV. Constructed by inserting 8.9 Kbp EcoRI fragment containing 3611-MSV
This clone transforms transfected 3T3 cells and induces sarcoma in NFS/N mice.
Includes about 50 bp of 5' and 1.3 kb of 3' flanking cellular (rat FRE-3A) sequences.
The v-raf gene is contained in a 1.5 kb XhoI/BglII fragment of the insert.
Classification Retroviridae, Gammaretrovirus, Mammalian virus group
References

Rapp UR, et al. New mammalian transforming retrovirus: demonstration of a polyprotein gene product. J. Virol. 45: 914-924, 1983. PubMed: 6300462

Bonner T, et al. The human homologs of the raf (mil) oncogene are located on human chromosomes 3 and 4. Science 223: 71-74, 1984. PubMed: 6691137

Rapp UR, et al. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Natl. Acad. Sci. USA 80: 4218-4222, 1983. PubMed: 6308607

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