Escherichia coli (Migula) Castellani and Chalmers (ATCC® 43895)

Strain Designations: CDC EDL 933  /  Type Strain: no  /  Biosafety Level: 2

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Classification Enterobacteriaceae, Escherichia
Strain Designations CDC EDL 933
Application
Media testing
Produces Shiga-like toxin I
Produces Shiga-like toxin II
Quality control strain
Quality control strain for Difco products
Isolation
Raw hamburger meat implicated in hemorrhagic colitis outbreak
Type Strain no
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype O157:H7
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O157:H7
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor CDC
Isolation
Raw hamburger meat implicated in hemorrhagic colitis outbreak
References

Strockbine NA, et al. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect. Immun. 53: 135-140, 1986. PubMed: 3522426

Wells JG, et al. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18: 512-520, 1983. PubMed: 6355145

Marques LR, et al. Production of Shiga-like toxin by Escherichia coli. J. Infect. Dis. 154: 338-341, 1986. PubMed: 3522760

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Cross References

Nucleotide (GenBank) : X96953 E. coli espB gene.

Nucleotide (GenBank) : AX110209 Sequence 942 from Patent WO0123604.

Nucleotide (GenBank) : AX110240 Sequence 973 from Patent WO0123604.

Nucleotide (GenBank) : L31880 Escherichia coli ribosomal RNA sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation