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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 2.8499999046325680 Vector: pRX-3 (plasmid) Promoters: Promoter trpE Construction: pBR322, trpE, M13mp13 polylinker Marker(s):ampR Construct size (kb): 2.849999904632568 Features: marker(s): ampR
promoter: trpE
replicon: pMB1
MCS: EcoRI...ClaI
terminator: unknown terminator
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Applications
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expression vector vector permitting construction of fusion proteins
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--2.85; ClaI--2.85. When Escherichia coli CAG-456 (a protease deficient host) is used, this vector yields large amounts of the fusion protein (100 mg/liter or 45% of the soluble protein). One of a series of plasmid expression vectors ( ATCC 37677 to 37679) representing multiple reading frames for expression from the trpE promoter. The fusion proteins produced contain less than 25 amino acids of the trpE protein. This vector contains the trpE operon and 51 bp of the trpE gene, followed by a 12-mer EcoRI linker and the M13mp13 multiple cloning site (MCS). There is a TGA stop codon in frame from the GAA of the EcoRI site 33 bp beyond the MSC ClaI site.
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References
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Rimm DL, Pollard TD. New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli. Gene 75: 323-327, 1989. PubMed: 2653968
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