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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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pSV2RbetaG [pSV2beta-globin]
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Vector Information
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Size (kb): 4.7199997901916500 Vector: pSV2RbetaG (plasmid) Promoters: Promoter SV40 early Marker(s):ampR Construct size (kb): 4.71999979019165 Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
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Applications
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expression vector shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): HindIII/BglII--4.23, 0.49; PvuII/PstI--2.22, l.55, 0.96; PstI--3.76, 0.96. Expresses beta-globin in mammalian cells. This plasmid includes the SV40 promoter (SV nt 270-5171), rabbit beta globin (487 bp), the SV40 splice site (SV nt4100-4710), the SV40 poly(A) (SV nt 1782-2770) and pBR322 (pBR nt 2067-4362).
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References
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Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549
Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915
Paul Berg, personal communication
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Shipping Information
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Distributed: freeze-dried
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