pBLCAT3 (ATCC® 37528)

Applications: vector for other usesvector permitting construction of fusion proteins  /  Depositors: B Luckow, G Schutz

Permits and Restrictions

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Designations pBLCAT3
Depositors B Luckow, G Schutz
Biosafety Level 1
Vector Information
Size (kb): 4.3400001525878910
Vector: pBLCAT3 (plasmid)
Promoters: Promoter none
Construction: pUC18, SV40, cml
Marker(s):ampR
Construct size (kb): 4.340000152587891
Features: marker(s): ampR
promoter: none
replicon: pMB1
Applications
vector for other uses
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; BglII--4.4; ClaI--uncut.
Developed to simplify construction of hybrid CAT genes.
The ClaI site appears to have been destroyed during cloning.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Luckow B, Schutz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15: 5490, 1987. PubMed: 3037497

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
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