pRSVneo (ATCC® 37198)

Other IDs:

Nucleotide (GenBank) : VB0064 pRSVNeo cloning vector for high efficiency gene transfer into mammalian cells.

 /  Applications: co-transfection marker aminoglycoside phosphotransferase kanamycin resistance, neomycin resistanceshuttle vector  /  Depositors: BH Howard

Permits and Restrictions

View Permits

Designations pRSVneo
Depositors BH Howard
Other IDs

Nucleotide (GenBank) : VB0064 pRSVNeo cloning vector for high efficiency gene transfer into mammalian cells.

Biosafety Level 1
Vector Information
Size (kb): 5.7360000610351560
Vector: pRSVneo (plasmid)
Promoters: Promoter RSV LTR
Construction: pRSVcat, pSV2neo
Marker(s):G418R,ampR,neoR
Construct size (kb): 5.736000061035156
Features: marker(s): ampR
marker(s): neoR, G418R
promoter: RSV LTR
replicon: SV40
replicon: pMB1
restriction site: HindIII
Applications
co-transfection marker aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
shuttle vector
Comments
Escherichia coli/mammalian cell culture vector with selectable markers in both systems.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985

Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map