pRSVneo (ATCC® 37198)

Other IDs:

Nucleotide (GenBank) : VB0064 pRSVNeo cloning vector for high efficiency gene transfer into mammalian cells.

 /  Applications: co-transfection marker aminoglycoside phosphotransferase kanamycin resistance, neomycin resistanceshuttle vector  /  Depositors: BH Howard

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Designations pRSVneo
Other IDs

Nucleotide (GenBank) : VB0064 pRSVNeo cloning vector for high efficiency gene transfer into mammalian cells.

Depositors BH Howard
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.7360000610351560
Vector: pRSVneo (plasmid)
Promoters: Promoter RSV LTR
Construction: pRSVcat, pSV2neo
Marker(s):G418R,ampR,neoR
Construct size (kb): 5.736000061035156
Features: marker(s): ampR
marker(s): neoR, G418R
promoter: RSV LTR
replicon: SV40
replicon: pMB1
restriction site: HindIII
Applications
co-transfection marker aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
shuttle vector
Comments
Escherichia coli/mammalian cell culture vector with selectable markers in both systems.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985

Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map