Thermus thermophilus (Oshima and Imahori) Williams et al. (ATCC® 27634)

Strain Designations: HB8  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Thermus thermophilus Oshima and Imahori
Strain Designations HB8
Produces D-xylose isomerase
Produces fructose-1,6-bisphosphatase
Produces phosphofructokinase
Produces phosphoglucomutase
Produces restriction endonuclease TtHB8I
Produces thermostable D-xylose isomerase
Hot spring, Japan
Type Strain yes
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Thermus thermophilus HB8 genomic DNA, complete genome.

Type Strain yes
Heat stability of tRNA and ribosomes
Respiratory proteins
Iron-sulfur protein
Resistance to heavy metal ions
Genome sequenced strain
Medium ATCC® Medium 697: Thermus medium
Growth Conditions
Temperature: 70°C to 75°C
Atmosphere: Aerobic
Name of Depositor T Oshima
Hot spring, Japan

Chiong M, et al. Resistance of Thermus spp. to potassium tellurite. Appl. Environ. Microbiol. 54: 610-612, 1988.

Lehmacher A, Bisswanger H. Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8. J. Gen. Microbiol. 136: 679-686, 1990.

Yoshida M, et al. The thermostable allosteric enzyme: phosphofructokinase from an extreme thermophile. Biochem. Biophys. Res. Commun. 43: 36-39, 1971. PubMed: 4252961

Yoshida M, Oshima T. The thermostable allosteric nature of fructose 1,6-diphosphatase from an extreme thermophile. Biochem. Biophys. Res. Commun. 45: 495-500, 1971. PubMed: 4334342

Sato S, et al. A DNA methylase from Thermus thermophilus HB8. J. Biochem. 88: 737-747, 1980. PubMed: 6448253

. . J. Gen. Appl. Microbiol. 17: 513-517, 1971.

Degryse E, et al. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch. Microbiol. 117: 189-196, 1978. PubMed: 678024

Oshima T, Imahori K. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24: 102-112, 1974.

Williams RA, et al. DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori) [published erratum appears in Int. J. Syst. Bacteriol. 46: 629, 1996]. Int. J. Syst. Bacteriol. 45: 495-499, 1995. PubMed: 8590676

Venegas A, et al. A rapid procedure for purifying a restriction endonuclease from Thermus thermophilus (Tth I). FEBS Lett. 109: 156-158, 1980. PubMed: 6243575

Yoshida T, et al. Respiratory proteins from the extremely thermophilic aerobic bacterium, Thermus thermophilus. Purification procedures for cytochromes c552, c555,549, and c1aa3 and chemical evidence for a single subunit cytochrome aa3. J. Biol. Chem. 259: 112-123, 1984. PubMed: 6323398

Fee JA, et al. Purification and characterization of the Rieske iron-sulfur protein from Thermus thermophilus. Evidence for a [2Fe-2S] cluster having non- cysteine ligands. J. Biol. Chem. 259: 124-133, 1984. PubMed: 6323399

Yoshizaki F, et al. Studies on phosphoglucomutase from an extreme thermophile, Flavobacterium thermophilum HB8. I. Thermostability and other enzymatic properties. J. Biochem. 69: 1083-1089, 1971. PubMed: 5091939

Sato S, Shinomiya T. An isochizomer of TaqI from Thermus thermophilus HB8. J. Biochem. 84: 1319-1321, 1978. PubMed: 730757

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

type strain

hot spring

Cross References

Nucleotide (GenBank) : M35674 T.thermophilus 23S ribosomal RNA gene.

Nucleotide (GenBank) : X58342 T.thermophilus 16S ribosomal RNA, part.

Nucleotide (GenBank) : M33159 T.thermophilus insertion sequences Is1000A and Is1000B.

Nucleotide (GenBank) : X06657 Thermus thermophilus HB8 tuf gene for elongation factor Tu (EF-Tu).

Nucleotide (GenBank) : U17352 Thermus aquaticus L-glutamine:D-fructose-6-P amidotransferase (glmS) gene, complete cds and s-layer P100 (slp) gene, partial cds.

Nucleotide (GenBank) : AP008226 Thermus thermophilus HB8 genomic DNA, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation