Deinococcus radiophilus (Lewis) Brooks and Murray (ATCC® 27603)

Strain Designations: RBD [ATCC 27602, ATCC 27604, CCM 2564, NCIB 10648, NCTC 10785, UWO 1055]  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Micrococcus radiophilus Lewis
Strain Designations RBD [ATCC 27602, ATCC 27604, CCM 2564, NCIB 10648, NCTC 10785, UWO 1055]
Application
Produces restriction endonuclease DraI
Produces restriction endonuclease DraII
Produces restriction endonuclease DraIII
Isolation
Irradiated Bombay duck, Harpodon nehereus
Type Strain yes
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Medium Medium 679: TGYM medium
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor NF Lewis
Isolation
Irradiated Bombay duck, Harpodon nehereus
References

Curr. Sci. 42: 504, 1973.

Lewis NF. Studies on a radio-resistant coccus isolated from Bombay duck (Harpodon nehereus). J. Gen. Microbiol. 66: 29-35, 1971. PubMed: 4934504

Brooks BW, Murray RG. Nomenclature for "Micrococcus radiodurans" and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Bacteriol. 31: 353-360, 1981.

. . FEBS Lett. 180: 219-223, 1985.

Lewis NF, Kumta US. Evidence for extreme UV resistance of Micrococcus sp. NCTC 10785. Biochem. Biophys. Res. Commun. 47: 1100-1105, 1972. PubMed: 5029860

Purvis IJ, Moseley BE. Isolation and characterisation of DraI, a type II restriction endonuclease recognising a sequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA. Nucleic Acids Res. 11: 5467-5474, 1983. PubMed: 6310500

Grosskopf R, et al. Two new restriction endonucleases DraII and DraIII from Deinococcus radiophilus. Nucleic Acids Res. 13: 1517-1528, 1985. PubMed: 2987827

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Cross References

Nucleotide (GenBank) : X52299 Deinococcus radiophilus 5S ribosomal RNA.

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation