Saccharomyces cerevisiae Meyen ex E.C. Hansen (ATCC® 204679)

Alternate State: Candida robusta Diddens et Lodder  /  Strain Designations: YPH499  /  Product Format: frozen

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Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations YPH499
Alternate State Candida robusta Diddens et Lodder
Application
Host strains for transformation, P. Hieter strains (BA1)
Biosafety Level 1
Product Format frozen
Type Strain no
Genotype MATa ura3-52 lys2-801(amber mutation) ade2-101(ochre mutation) trp1-delta63 his3-delta200 leu2-delta1 RefSikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436, (The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .)
Preceptrol® no
Mating Type a
Comments
Host strains for transformation, P. Hieter strains (BA1)
Medium Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Sequenced Data
Not available.
Name of Depositor YGSC
Special Collection Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--R. Sikorski and P. Hieter
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation