To prepare the recommended medium for NK-92® (ATCC® CRL-2407™)
cells, use Alpha MEM without ribonucleosides (Thermofisher 12000-063
with added 1.5 g/L sodium bicarbonate Sigma S5761), and supplement with:
0.2 mM Myo-inositol (final conc.) Sigma I-7508,
0.1 mM 2-mercaptoethanol (final conc.) Gibco 21985-023,
0.02 mM folic acid (final conc.) Sigma F-8758,
100 U/ml recombinant human IL-2 Peprotech 200-02 or Sigma I7908,
Fetal bovine serum to a final concentration of 12.5% FBS (ATCC® 30-2020™) (not heat-inactivated)
Horse serum to a final concentration of 12.5% Horse Serum (ThermoFisher 16050122; not heat-inactivated)
Note: Thermofisher 12000-063 is supplied as a powder. Add 1.5 g/L of sodium bicarbonate and reconstitute in water, then filter sterilize.
ATCC does not recommend using antibiotics/antimycotics, if antibiotics are required, 1% solution of Penicillin-Streptomycin Solution (ATCC® 30-2300™) is recommended. Use strict aseptic techniques when handling the cells.
NK-92® is a registered trademark of Nantkwest, Inc.
|Date Created||12/01/2019 04:53 PM
|Date Updated||02/04/2020 07:39 AM