Cryopreserved Ker-CT cells (ATCC® CRL-4048
™) should be quickly thawed at 37o
C in a water bath. After thawing, add the contents of the cryovial to a conical tube with 10mL prewarmed complete medium. Count the cells and seed at the recommended density.
DO NOT CENTRIFUGE at this point, as the cells are fragile after thawing and centrifugation may damage the cells.
After most cells attach to the culture vessel(s), which usually take about 6 hours to overnight, remove the medium with FBS and DMSO and replace with fresh complete growth medium. NOTE that during subculture, the cells can be centrifuged as recommended in the product sheet.
|Date Created||11/19/2013 11:45 AM
|Date Updated||03/27/2014 08:43 PM