To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves. If you experience any issues, please contact ATCC Customer Service at For Technical questions please contact Thank you for your understanding, patience and flexibility as ATCC does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers.


FAQ Home

Find Answers

Post-thaw yield and viability of ATCC® PCS-800-012 ™ Human Primary Bone Marrow CD34+ cells

What can I do to improve the post-thaw yield and viability of cryopreserved ATCC® PCS-800-012 ™, Human Primary Bone Marrow CD34+ cells?

We recommend the following thawing procedure:

  • Rapidly thaw the cryovial in a 37°C water bath (1-2 min). Remove the vial before all the ice is thawed.
  • Decontaminate the cryovial with 70% ethanol and move it to a biosafety cabinet and keep on ice.
  • While on ice, transfer contents of the vial to a 15mL conical tube.
  • Rinse the cryovial with 1mL of ice-cold thawing media (Hank's Balanced Salt Solution without Ca2+ or Mg2+ (ATCC® 30-2213 ™), supplemented with 10% Fetal Bovine Serum (ATCC® 30-2020 ™) and slowly transfer to the cells to the conical tube.
  • Slowly pipet 10X the vial volume of thawing media into the conical tube.
  • Take a sample for counting and viability assessment.
  • Centrifuge the cells at 4°C at 300 x g for 5 minutes to pellet cells.
  • Aspirate the supernatant carefully, leaving a few mLs behind.
  • Re-suspend cell pellet in the assay media of your choice.

While some reports (Ref) suggest increased viability using warm thawing media, ATCC has routinely achieved viability of over 80% as determined by trypan blue exclusion assays immediately post-thaw, using this protocol.

Date Created08/27/2014 07:14 AM
Date Updated01/12/2017 09:27 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. U-373 MG (ATCC® HTB-17)
  5. Converting TCID[50] to plaque forming units (PFU)