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Filter Size to Use for Hybricare 46-X
What size filter should one use to sterilize the Hybricare media (ATCC® 46-X ™)

Hybricare medium (ATCC® 46-X ™) is sold as a powder.  After hydration, sodium bicarbonate must be added and the medium sterile filtered through a 0.22 micron filter.  Given the number of components that are present in HybriCare, filtration will be slow using the small pore size.  Serum should be added aseptically after the filtration.
Date Created07/17/2012 07:16 PM
Date Updated06/16/2014 08:44 AM

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