To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >
X

FAQ's

FAQ Home

Find Answers

Filter Size to Use for Hybricare 46-X
What size filter should one use to sterilize the Hybricare media (ATCC® 46-X ™)

Hybricare medium (ATCC® 46-X ™) is sold as a powder.  After hydration, sodium bicarbonate must be added and the medium sterile filtered through a 0.22 micron filter.  Given the number of components that are present in HybriCare, filtration will be slow using the small pore size.  Serum should be added aseptically after the filtration.
Date Created07/17/2012 07:16 PM
Date Updated06/16/2014 08:44 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. Converting TCID[50] to plaque forming units (PFU)
  5. U-373 MG (ATCC® HTB-17)