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ATCC CRL-1660 culture conditions

What are the culture conditions of CRL-1660 cells?

CRL-1660 cells are not anchorage dependent. These cells can be subcultured by simply washing the flask surface with medium and pipetting a bit. Viable cells tend to float freely in the medium and may require 5 days in culture before even 60% confluency is reached. Because of the nature of these cells it is best to allow the cells at least 2 days of undisturbed incubation before assessing percent attachment and care should be taken not to discard viable cells during media changes performed during the first week. Even after the cells are well-attached, there will still be some viable floating cells.

Date Created12/18/2017 09:56 AM
Date Updated12/18/2017 09:57 AM

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