MRC-5 cells can be sensitive to DMSO and the cryoprotectant should be removed immediately upon thaw, by gentle centrifugation (125 × g for 10 minutes) in 9-10 mls of complete ATCC-formulated Eagle's Minimum Essential Medium, (ATCC® 30-2003
™) supplemented with 10% fetal bovine serum (ATCC® 30-2020
™). The MRC-5 fibroblast cell line was derived from normal lung tissue and the cells are capable of 42 to 46 population doublings before the onset of senescence.
|Date Created||02/21/2014 11:49 AM
|Date Updated||02/21/2014 11:51 AM