ATCC advises against the use of the MDA-MB-435S (ATCC® HTB-129) cell line as a model for original source material. Recent studies have generated questions about the origin of the parental cell line, MDA-MB-435, and by extension MDA-MB-435S and its derivatives listed below. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast (PubMed IDs: 10700174, 1510101, 15679052).
Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line (PubMed IDs: 12354931, 17004106).
Derivatives of MDA-MB-435S with identities in question are:
| Designation|| ATCC® Number|
| MDA-kb2|| CRL-2713|
| M4A4|| CRL-2914|
| M4A4 GFP|| CRL-2915|
| M4A4 LM3-2 GFP|| CRL-2916|
| M4Af LM3-4 CL16 GFP|| CRL-2917|
| NM2C5|| CRL-2918|
| NM2C5 GFP|| CRL-2919|
|Date Created||07/17/2012 07:16 PM
|Date Updated||07/25/2012 05:23 PM