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ATCC® CCL-92 vs. ATCC® CRL-1658

Are the 3T3-Swiss (ATCC® CCL-92 ™) cell line and the NIH/3T3 (ATCC® CRL-1658 ™) cell line the same? What does 3T3 mean?

No, 3T3-Swiss and NIH/3T3 are not the same cell line. There are many strains of Swiss mice. NIH Swiss is a specific strain of Swiss mice bred at the NIH. These are not inbred, nor are they completely outbred either.

ATCC® CCL-92 ™ is from Swiss albino mice while ATCC® CRL-1658 ™ was developed from NIH Swiss mice (the specific strain of Swiss at NIH). 3T3 is a designation that stands for passaging the cells 3 times/week at 1:3 (This is the method by which the cells were established).

Photomicrographs of ATCC® CCL-92 and ATCC® CRL-1658 are available in the product descriptions on the ATCC website.

Date Created07/17/2012 07:12 PM
Date Updated02/05/2013 03:27 PM

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