To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves. If you experience any issues, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you for your understanding, patience and flexibility as ATCC does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers.
X

FAQ's

FAQ Home

Find Answers

ATCC CCL-228 growth and morphology

What are the growth conditions and morphology of CCL-228 cells?

It is normal for many viable SW480 cells remain in suspension for the first few days post thaw, and be only loosely attached.  These cells are cultured in L-15 medium in atmospheric air conditions.  If it is not possible to dedicate an incubator to atmospheric air incubation, then tissue culture flasks with tightened, non-vented, plug seal caps can be used to prevent the CO2 atmosphere from entering the flasks.  When feeding with fresh medium these cells should be handled gently and any floating cells should be retained by gentle centrifugation and added back to the same flask with the adherent cells. The floating cells will eventually attach and grow well.  Discarding the floating population or separating into several flasks can make the culture too dilute, causing growth to slow or stop completely.  

Date Created12/18/2017 10:04 AM
Date Updated12/18/2017 10:04 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. Converting TCID[50] to plaque forming units (PFU)
  5. U-373 MG (ATCC® HTB-17)