It is normal for many viable SW480 cells remain in suspension for the first few days post thaw, and be only loosely attached. These cells are cultured in L-15 medium in atmospheric air conditions. If it is not possible to dedicate an incubator to atmospheric air incubation, then tissue culture flasks with tightened, non-vented, plug seal caps can be used to prevent the CO2 atmosphere from entering the flasks. When feeding with fresh medium these cells should be handled gently and any floating cells should be retained by gentle centrifugation and added back to the same flask with the adherent cells. The floating cells will eventually attach and grow well. Discarding the floating population or separating into several flasks can make the culture too dilute, causing growth to slow or stop completely.
|Date Created||12/18/2017 10:04 AM
|Date Updated||12/18/2017 10:04 AM