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Culture conditions for A-549 VIM RFP cells
What are the recommended culture conditions for A-549 VIM RFP cells compared to the parental A-549 cells?

The recommended culture medium for A-549 VIM RFP (ATCC® CCL-185EMT™) cells is F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004) supplemented with 10% Fetal Bovine Serum (FBS) (ATCC® 30-2020). Optionally you can add 10 µg/ml blasticidin to the culture medium although this is not required for this stable cell line. The parental A549 (ATCC® CCL-185) cells require a similar complete growth medium.

Date Created10/12/2017 11:40 AM
Date Updated10/12/2017 11:41 AM

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