FAQ Home

Find Answers

Culture conditions for A-549 VIM RFP cells
What are the recommended culture conditions for A-549 VIM RFP cells compared to the parental A-549 cells?

The recommended culture medium for A-549 VIM RFP (ATCC® CCL-185EMT™) cells is F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004) supplemented with 10% Fetal Bovine Serum (FBS) (ATCC® 30-2020). Optionally you can add 10 µg/ml blasticidin to the culture medium although this is not required for this stable cell line. The parental A549 (ATCC® CCL-185) cells require a similar complete growth medium.

Date Created10/12/2017 11:40 AM
Date Updated10/12/2017 11:41 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. U-373 MG (ATCC® HTB-17)
  5. Converting TCID[50] to plaque forming units (PFU)