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HTB-38 morphology and media
What is the morphology of ATCC® HTB-38 ™, HT-29 cells after initiation?

There may be many viable floating cells during the first week of recovery of HT-29 cells from cryopreservation.  When feeding the culture with fresh medium the floating cells should be retained by gentle centrifugation (125 × g for 10 minutes) and added back to the adherent population.  The cells will attach in patches as three-dimensional aggregates and as they divide and grow they will flatten into an epithelial cell monolayer. ATCC-formulated McCoy's 5a Medium Modified, (ATCC® 30-2007 ™) supplemented with 10% fetal bovine serum (ATCC® 30-2020 ™) is recommended for culture of these cells.

Date Created02/21/2014 11:54 AM
Date Updated02/21/2014 11:55 AM

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