There may be many viable floating cells during the first week of recovery of HT-29 cells from cryopreservation. When feeding the culture with fresh medium the floating cells should be retained by gentle centrifugation (125 × g for 10 minutes) and added back to the adherent population. The cells will attach in patches as three-dimensional aggregates and as they divide and grow they will flatten into an epithelial cell monolayer. ATCC-formulated McCoy's 5a Medium Modified, (ATCC® 30-2007 ™) supplemented with 10% fetal bovine serum (ATCC® 30-2020 ™) is recommended for culture of these cells.
|Date Created||02/21/2014 11:54 AM
|Date Updated||02/21/2014 11:55 AM