Vacuoles are usually a sign of stress. Some of the causes of stress are lack of glutamine in the medium, addition of anti-fungal agents, improper CO2 environment for the sodium bicarbonate concentration in the medium or nutrient depletion of the medium.
Be careful, however, vacuolar appearance can be normal for some cell lines, especially those that have lipid droplets (vacuoles are normal for ATCC® HB-8065 ™ and for ATCC® CL-173 ™ after differentiation).
|Date Created||07/17/2012 07:12 PM
|Date Updated||02/06/2013 12:09 PM