Thaw the vial by gentle agitation in a 37°C water bath for approximately 2 minutes.
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol.
Transfer the vial contents to a centrifuge tube containing 9.0 mL of complete culture medium and centrifuge the cell suspension at approximately 125 x g for 5 minutes.
Discard the supernatant and resuspend the cells in fresh growth medium.
Count the cells and seed it at 4 - 6 x 103 cells/cm2 in culture vessel.
Incubate the culture at 37°C in a suitable incubator with a 5% CO2/95% air atmosphere.
|Date Created||02/13/2014 12:37 PM
|Date Updated||02/13/2014 12:42 PM