FAQ Home

Find Answers

Transfection media concerns
Should I use serum-free medium during transfection?

  • TransfeX  has been optimized for intracellular delivery of nuclei acids into cultured mammalian cells in the presence of serum. However, culture medium containing polyanions such as heparin, heparin sulfate or dextran sulfate can inhibit transfection. Medium containing these chemicals should not be used for transfection. However, the medium can be replaced with medium containing the polyanions 24hours post-transfection.
Date Created10/15/2013 03:39 PM
Date Updated03/27/2014 08:42 PM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. U-373 MG (ATCC® HTB-17)
  5. Converting TCID[50] to plaque forming units (PFU)