To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at For Technical questions please contact Thank you.

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >


FAQ Home

Find Answers

Dissociation reagents used during subculturing ATCC PCS-201-012

Are there specific reagents  for subculturing ATCC PCS-201-012?

For subculturing ATCC® PCS-201-012 the following reagents should be used: Trypsin-EDTA for primary cells (ATCC® PCS-999-003) and Trypsin Neutralizing Solution (ATCC® PCS-999-004).  The complete media for these primary cells may not fully neutralize the dissociation solution because it can be serum-free or low in FBS.                                                                                                                                                                                                                                                                                                                                                                                                

Date Created08/01/2019 08:35 AM
Date Updated08/01/2019 10:35 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. Converting TCID[50] to plaque forming units (PFU)
  5. U-373 MG (ATCC® HTB-17)