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Cells contain Human papillomavirus (HPV)
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. Exp. Biol. Med. 89: 362-364, 1955. PubMed: 13254761
Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740
Salzman NP. Animal Cell Cultures. Science 133:1559-65. 1961 PubMed: 13745868
Eagle H, Foley GE. Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents. Cancer Res. 18: 1017-1025, 1958. PubMed: 13596943
Foley GE, Handler AH. Differentiation of “Normal” and Neoplastic Cells Maintained in Tissue Culture by Implantation into Normal Hamsters. Proc Soc Exp Biol Med 94: 661-664 1957. PubMed: 13431913