Human adenovirus 12 (ATCC® VR-863)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus A  /  Product Format: frozen

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Classification Adenoviridae, Mastadenovirus, Human adenovirus A
Deposited As Adenovirus type 12
Agent Human adenovirus 12
Strain Huie
Applications
Produces tumors at sites of inoculation in newborn hamsters
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
May require passage with large inocula before satisfactory titers are obtained. Recognized reference strain: Pereira, H.G. et al., Virology 20:613, 1963.
Derived from NIAID V-212-001-014. HA activity has not been demonstrated. Produces tumors at sites of inoculation in newborn hamsters (s.c., i.c., intrathoracic).
Effect on Host
CPE; refractile rounding and sloughing
Recommended Host
KB (ATCC® CCL-17™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

Incubation: 2 to 3 days at 37°C, a 5% CO2 in air atmosphere is recommended

Effect on Host
CPE; refractile rounding and sloughing
Name of Depositor NIH/NIAID
Source Stool from case of suspected poliomyelitis, Massachusetts
References

Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation