CEM-CM3 (ATCC® TIB-195)

Organism: Homo sapiens, human  /  Cell Type: T lymphoblast  /  Disease: acute lymphoblastic leukemia

Organism Homo sapiens, human
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphoblastic leukemia
Age 4 years
Gender female
Ethnicity Caucasian
Applications
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
The line has been used as a fusion partner in the production of human-human T cell hybridomas.
Storage Conditions liquid nitogen vapor phase
Derivation
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
Clinical Data
female
Caucasian
4 years
Comments
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
The line has been used as a fusion partner in the production of human-human T cell hybridomas.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.015 mg/ml 8-azaguanine, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 10,12
D5S818: 12,13
D7S820: 10,13
THO1: 6,7
TPOX: 8
vWA: 18,19
Name of Depositor MK Cathcart
References

Cathcart MK, Murakami MNonspecific suppression of antibody synthesis by products of human T lymphocyte hybridomas. Use of a newly selected parent fusion lineIn: Cathcart MK, Murakami MT cell hybridomasBoca Raton, FLCRC Presspp. 227-236, 1985

. Human T lymphocyte hybridomas: production of nonspecific suppressor factors of antibody synthesis. Kyoto, Japan: 5th International Congress of Immunology; 1983.

. . Clin. Res. 31: 734A, 1983.

Basic Documentation
References

Cathcart MK, Murakami MNonspecific suppression of antibody synthesis by products of human T lymphocyte hybridomas. Use of a newly selected parent fusion lineIn: Cathcart MK, Murakami MT cell hybridomasBoca Raton, FLCRC Presspp. 227-236, 1985

. Human T lymphocyte hybridomas: production of nonspecific suppressor factors of antibody synthesis. Kyoto, Japan: 5th International Congress of Immunology; 1983.

. . Clin. Res. 31: 734A, 1983.