pGRN145 plasmid in E. coli GC10 (ATCC® MBA-141)

Depositors: Geron Corporation

Permits and Restrictions

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Designations pGRN145 plasmid in E. coli GC10
Depositors Geron Corporation
Applications Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit)
Vector
Total size (kb): 14.002
Bacterial selection marker: cmlR

 

Features:
hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
MPSV: 3643-4119 (promoter)
Ori: 12165-12747

 

Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.

Insert
Gene: Telomerase reverse transcriptase (TERT)
Source: Human
Insert size (kb): 3.398
GenBank accession: NM_003219
Media ATCC® Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen glycerol stock of E. coli GC10 containing the plasmid
Bascic Documentation
Restrictions

For commercial accounts, this cell line is only distributed under the terms of a fully signed and executed ATCC® Material Transfer Agreement and Addendum. If the commercial account is screening per completed Addendum, the recipient will be required to pay a Screening Fee (ATCC® ACS-2103™) of $4000.00.

Screening Use is defined as use of Biological Material in small molecule and biologic drug discovery, including initial target identification and validation, assay development, high throughput screening, hit identification, lead optimization, and selection of candidates for clinical development.

If the commercial account is not screening per the completed Addendum, the recipient will not be required to pay a Screening Fee.

In addition to the foregoing, this product's use is governed by the following Limited Use License. 

      1. This product may not be transferred to any third parties.
      2. This product may be used solely for internal scientific research (the “Field of Use”).  The Field of Use expressly excludes the use of the product:
          1. in a product for therapeutic or diagnostic use;
          2. in human clinical research;
          3. in the manufacture of a product intended for sale or of a component or intermediate of such a product; or
          4. in the discovery, research, development, manufacture or sale of a product that:
            1. acts by detecting or measuring telomere length, telomerase activity, telomerase RNA, or TERT mRNA or protein;
            2. acts through a response to the presence or absence of: (1) telomerase RNA, the gene encoding telomerase RNA or its promoter sequence; (2) the telomerase catalytic protein, the gene or mRNA encoding the telomerase catalytic protein, or its promoter sequence.  This includes, without limitation, a product that triggers an immune response to telomerase or any component thereof, or targets cells for a cytotoxic effect through the expression of a gene controlled by a telomerase promoter; or
            3. acts by modulating telomerase activity or telomere length (including, without limitation, inhibiting or activating telomerase).

Any use of the product outside of the Field of Use requires a separate license.  For information on obtaining a license to use this product for purposes other than those permitted in the Limited Use License, please contact ATCC at licensing@atcc.org.


Attachments MSDS
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