(CRL-8858)

U.S. Patent Number
Biosafety Level 2 CELLS CONTAIN RETROVIRUS, RECOMBINANT

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The cells contain a recombinant retrovirus genome that contains sequences coding for hGH and neomycin resistance.
The cells produce and secrete hGH.
Recovery of the cells from the frozen state, and subsequent proliferation, may be enhanced by the use of a feeder layer of irradiate or mitomycin C treated 3T3 cells.
IRR-3T3 (ATCC 48-X) may be used as a feeder layer for this cell line.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Basic Documentation
References

Morgan JR, Mulligan RC. Introduction and expression of foreign genetic material in epithelial cells. US Patent 4,868,116 dated Sep 19 1989

Morgan JR, Mulligan RC. In vivo introduction and expression of foreign genetic material in epithelial cells. US Patent 4,980,286 dated Dec 25 1990

Allen-Hoffmann BL, Rheinwald JG. Polycyclic aromatic hydrocarbon mutagenesis of human epidermal keratinocytes in culture. Proc. Natl. Acad. Sci. USA 81: 7802-7806, 1984. PubMed: 6440145