OKT 9 (ATCC® CRL-8021)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia,Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia,Leukemia
Applications
The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor.
Derivation
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor
Comments
Animals were immunized with human T cell leukemia cells (acute lymphoblastic leukemia).
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Ortho Pharmaceutical Corporation
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Kung PC, Goldstein G. Monoclonal antibody to a human early thymocyte antigen and methods of preparing same. US Patent 4,364,934 dated Dec 21 1982

Sutherland R, et al. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation- associated receptor for transferrin. Proc. Natl. Acad. Sci. USA 78: 4515-4519, 1981. PubMed: 6270680

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kung PC, Goldstein G. Monoclonal antibody to a human early thymocyte antigen and methods of preparing same. US Patent 4,364,934 dated Dec 21 1982

Sutherland R, et al. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation- associated receptor for transferrin. Proc. Natl. Acad. Sci. USA 78: 4515-4519, 1981. PubMed: 6270680