Complete Growth Medium
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The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
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Subculturing
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Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium as cell density increases.
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Cryopreservation
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Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
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