Derivation
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EpH4-Ev was produced by transfecting parental EpH4, a mouse mammary epithelial cell line, with an empty expression vector carrying a puromycin resistance gene. Stable clones were isolated by selection in medium containing 1.2 µg/mL puromycin.
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Genes Expressed
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puromycin resistance gene, expressed
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Comments
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EpH4-Ev was produced by transfecting parental EpH4, an immortalized mouse mammary epithelial cell line, with an empty expression vector carrying a puromycin resistance gene. Stable clones were isolated by selection in medium containing 1.2 mg/mL puromycin.
The EpH4 cell line presents an attractive model in which to study transformation because the cells exhibit morphological and functional characteristics typical of normal mammary epithelial cells. This cell line has been used to generate constitutively activated MEK1 transformed cell line B-MEKDD 116 (ATCC CRL-3069). There are three additional related cell lines: EpH 1424 (ATCC CRL-3071), EpH4 1424.1 (ATCC CRL-3209), EpH4 1424.2 (ATCC CRL-3210).
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