SU-DHL-10 (ATCC® CRL-2963™) Organism: Homo sapiens, human / Cell Type: B lymphocyte / Tissue: lymph node Derived from Metastatic Site: peritoneal effusion / Disease: Large Cell Lymphoma General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Tissue lymph node Derived from Metastatic Site: peritoneal effusion Cell Type B lymphocyte Product Format frozen Morphology lymphoblast-like Culture Properties suspension, multicellular aggregates Biosafety Level 1 Disease Large Cell Lymphoma Age 25 years Gender Male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Images Clinical Data 25 years Caucasian Male Comments ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 2.0 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium renewal: every 2 to 4 days Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37.0°CAtmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile D5S818: 11, 12 D13S317: 10, 11 D7S820: 8, 11 D16S539: 10, 11 vWA: 17 THO1: 8, 9.3 TPOX: 8 CSF1PO: 10, 13 Amelogenin: XY Isotype IgG lambda Name of Depositor A Epstein Year of Origin 1978 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Other Documentation Cell Micrograph