Derivation
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The BCL2 (S70A) Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 mutated ORF insert and a neomycin-resistant gene (Parental vector: Addgene # 3349). Bcl2 (S70A) stable neomycin (Neo)-resistant single-cell originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.
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Genes Expressed
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Neomycin resistance gene, expressed; BCL2 (S70A) mutant, over-expressed, verified at ATCC
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Comments
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The BCL2 (S70A) Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL2 mutated ORF insert and a neomycin-resistant gene (Parental vector: Addgene # 3349). The BCL2 (S70A) Jurkat cells contain the BCL2 ORF with a sub-mutation at Ser70, containing an amino-acid substitution to Ala70. BCL2 (S70A) stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days. The BCL2 (S70A) Jurkat cell line over-expresses the S70A-mutated form of Bcl-2.
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