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HCC1187 (ATCC® CRL-2322)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: mammary gland; breast  /  Disease: TNM stage IIA, grade 3, primary ductal carcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue mammary gland; breast
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties mixed, adherent and suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease TNM stage IIA, grade 3, primary ductal carcinoma
Age 41 years adult
Gender female
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype multiploid; cell population has several ploidy indices
Images CRL-2322 Micrograph
This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish.
Clinical Data
41 years

Receptor Expression
progesterone receptor, negative
Oncogene her2/neu -, p53 +
Cellular Products
Epithelial glycoprotein 2 (EGP2)
cytokeratin 19
The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.
The cells are poorly differentiated.

An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323.

HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of Her2-neu; they overexpress p53. The cells are negative for expression of progesterone receptor (PR).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. Cultures can be maintained by the addition of fresh medium to the flask, or centrifuging and removing the spent medium, resuspending the cell pellet in fresh medium and returning the resuspended cells to the flask.

To subculture the attached cells, remove and reserve the suspended cells (recover the suspended cells by centrifugation if desired). Rinse the monolayer with fresh 0.25% trypsin-0.53 mM EDTA, neutralize with an equal volume complete medium and centrifuge. Remove supernatant and resuspend cell pellet with complete medium and pool into reserved suspended cells. Add appropriate aliquots of the pooled cell suspension to new culture vessels.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 10
D5S818: 12
D7S820: 8,11
TH01: 6
vWA: 19
Name of Depositor AF Gazdar, AK Virmani

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email, or Fax: (214) 951-0935.


Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771